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GraphPad Software Inc sigmoid 4pl interpolation
Sigmoid 4pl Interpolation, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GraphPad Software Inc sigmoid 4pl interpolation graphpad prism 9.0
A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.
Sigmoid 4pl Interpolation Graphpad Prism 9.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sigmoid 4pl interpolation graphpad prism 9.0/product/GraphPad Software Inc
Average 90 stars, based on 1 article reviews
sigmoid 4pl interpolation graphpad prism 9.0 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

Journal: bioRxiv

Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses

doi: 10.1101/2023.07.14.549076

Figure Lengend Snippet: A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

Article Snippet: The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

Techniques: Neutralization, Infection, Incubation, Luciferase, Inhibition