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The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid <t>4PL</t> <t>interpolation</t> with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
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sigmoid 4pl interpolation graphpad prism 9.0  (GraphPad Software Inc)
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A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.
Sigmoid 4pl Interpolation Graphpad Prism 9.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Journal: bioRxiv

Article Title: Development and Characterization of Recombinant Vesicular Stomatitis Virus (rVSV)-based Bivalent Vaccine Against COVID-19 Delta Variant and Influenza Virus

doi: 10.1101/2021.12.14.472657

Figure Lengend Snippet: The neutralization titers (50% inhibition) in immunized mice against SPΔC wt -Luc-PVs (A), SPΔCdelta-Luc-PVs (B), SPΔC beta’ -Luc-PVs (D) and SPΔC b.1.617 -Luc-PVs (E) after 28 days of prime-boost vaccination with different bivalent vaccines. VSV-G-Luc-PVPs (C) was used as negative control. The mouse serum of each immunization group collected at day 28 were pooled together, 2× serially diluted and incubated with different Luc-PVs (∼10 4 RLU). Then, the mixtures were added in A549 ACE2 . cell cultures and the infection of PVs was determined by Luciferase assay at 48∼66 hrs post infection. The percentage of infection was calculated compared with no serum control and neutralizing titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. The neutralizing titers of V-EM2/SPΔC1 immunized mouse sera against different SPΔC-Luc-PVs were listed (F). Data represent Mean ±SD and were obtained from over three independent experiments. Statistical significance was determined using ordinary one-way ANOVA test and Turkey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Article Snippet: The endpoint titer is designated as the reciprocal of the highest dilution of a serum that has an OD450 above the cutoff (10× negative control) and is calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

Techniques: Neutralization, Inhibition, Vaccines, Negative Control, Incubation, Infection, Luciferase, Control

A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

Journal: bioRxiv

Article Title: Oral immunization with rVSV bivalent vaccine elicits protective immune responses, including ADCC, against both SARS-CoV-2 and Influenza A viruses

doi: 10.1101/2023.07.14.549076

Figure Lengend Snippet: A) The duration of anti-SARS-CoV-2 RBD (endpoint titers) in the i.n. immunized mice sera after booster immunization (3wpB and 5wpB) were measured. B) The neutralization titers (50% inhibitory dose, ID 50 ) in immunized mice sera (3wpB) against pseudovirus PV-Luc-SP Delta infection were determined. The serially diluted mouse sera were incubated with PV-Luc-SP Delta (∼10 4 RLU) and then, the mixtures (PV + Sera) were used to inoculate A549 ACE2 cells. The infection of PV was determined by luciferase assay at 48∼66 hrs post-infection. The percentage of infection was calculated compared with no serum control. The 50% inhibition dose (ID 50 ) neutralizing Ab titers were calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0, as described in Materials and Methods. C) The duration of neutralizing Ab ID 50 titers in the i.n. immunized mice sera collected at 3wpB and 5wpB. Data represent mean ±SEM. Statistical significance was determined using one-way ANOVA test and Tukey’s test. *, P < 0.05; **, P < 0.01; ***, P < 0.001. wpB, week post Boost.

Article Snippet: The ID 50 was calculated by using sigmoid 4PL interpolation with GraphPad Prism 9.0.

Techniques: Neutralization, Infection, Incubation, Luciferase, Inhibition